Big Data, Big Problems.
It is with deep regret and following much contemplation that we need to cancel the inaugural light sheet workshop planned for this July. We absolutely did not want to do this but challenges in arranging travel, housing (summer housing is shutdown), live animals etc are proving too significant to overcome at this time.
While we expect the current crisis will have abated by the end of July, we feel the current climate is not conducive to the energy and interest we want to generate for the first US based light sheet workshop.
We are truly disappointed that we have to cancel the event.
We do plan on holding this workshop next year, during a same timeframe, exact dates to be determined in the near future.
Wishing wellness and safety to all of you and yours
Acquiring data using light-sheet and other technologies is hard enough.
This workshop has three main goals:
Teaching you how to prepare your sample
Showing you what technique works best for what size sample.
Helping you determine how to process and analyze your data.
Big Data, Big Problems
Big Data, Big problems was conceived during a panel discussion at a North Atlantic Microscopy Society (NAMS) meeting. Scientific attendees voiced the need for an extended light-sheet workshop in the U.S..
Big Data, Big Problems 2019
Amy Elliot National Institutes of Health
Holly Gibbs Texas A&M
Elizabeth Hillman Columbia University
Jan Huisken Morgridge Institute for Research
Gary Laevsky Princeton University
Talley Lambert Harvard University
Wesley Legant University of North Carolina
Paul Maddox University of North Carolina
Alison North The Rockefeller University
Doug Richardson Harvard University
Hari Shroff National Institutes of Health
Claudette St. Croix University of Pittsburgh
Sebastian Streichan University of California Santa Barbara
Simon Watkins University of Pittsburgh
Big Data; Big Questions
1:30-2:30 Optical principals SimonWatkins
2:30-3:30 Fundamentals of optical sectioning Simon Watkins
3:30-4:30 Fluorescent probes/proteins Claudette St. Croix
4:30-6:00 3D imaging options Alison North
8-? Instrument tour (pick your imaging system)
9-10 What is a light-sheet? Talley Lambert
10-11 Detectors Simon Watkins
11-12 What kinds of light-sheet exist? Talley Lambert
1-2 Match sample to system/application Gary Laevsky
2-3 Clearing Techniques Douglass Richardson
3-6 Lab: Sample prep
9-9:30 Elizabeth Hillman (SCAPE)
9:30-10:00 Sebastian Streichan (MuVi)
10-10:30 Paul Maddox (TILT)
10:45-11:15 Amy Elliot (diSPIM)
11:15-11:45 Jan Huisken (Flamingo)
11:45-12:15 Wesley Legant (Lattice)
1-5 Analysis of collected datasets
5-6:30 Light-sheet applications
Jared Toettcher (fly embryo/monolayer)
Eszter Posfai (mammalian embryo)
Kelly Seagraves (whole mouse brain imaging)
7:30-? Acquisition (compare systems used before/new technology)
9-10 Data Handling Douglas Richardson
10-11 3D data visualization Holly Gibbs
11-12 Data Handling Douglas Richardson
1-6 Canned datasets/analysis
6-9 Closing reception/talk Jan Huisken
9-11 Student presentations
10-11 Funding and futures SimonWatkins
11-12 Closing talk Hari Schroff
12-1 Box lunch/Departure